DECADES OF RESEARCH

To view more about each finding, click on any of the timeline discoveries below.

1997

KLOTHO GENE DISCOVERED BY JAPANESE GROUP KUROSU ET AL

The aging-suppressor gene that was identified by Kurosu et al in 1997 was named Klotho. The overexpression of the mouse α-Klotho gene slowed the aging process and extended the life span by 20–30%. View official Study

2005

KUROSU ET AL DEMONSTRATED THAT THE OVEREXPRESSION OF KLOTHO PROTEIN INHIBITED INSULIN (INSULIN GROWTH FACTOR 1) SIGNALING AND INCREASED LIFESPAN

Suppression of aging in mice by the hormone Klotho: Klotho Protein functions as a circulating hormone that binds to a cell-surface receptor and represses intracellular signals of insulin and insulin-like growth factor 1 (IGF1), an evolutionarily conserved mechanism for extending life span. Inhibiting insulin IGF1 signaling activates transcription factors that increase cellular protection against oxidative stress and may contribute to suppression of aging. View official Study

2006

SCIENTISTS CONTINUE TO STUDY LINKS BETWEEN KLOTHO & GROWTH FACTOR SIGNALING

REGULATION OF FIBROBLAST GROWTH FACTOR-23 SIGNALING BY KLOTHO: The Klotho Protein directly binds to multiple FGF receptors (FGFRs). View official Study

2008

THE PHYSIOLOGICAL RELEVANCE OF AMPK IS DEMONSTRATED AND ITS ROLE IN THE REGULATION OF ENERGY HOMEOSTASIS IS ESTABLISHED

AMPK: a metabolic gauge regulating whole body energy homeostasis: Science accumulates evidence that AMPK is involved in the regulation of energy balance at the whole-body level by responding to hormones and nutrient signals, which leads to changes in energy homeostasis. View official Study

REVOLUTIONARY STUDIES

Studies performed by Gail Humble M.D. & the Klotho Skin team.

2018

DISCOVERY OF AGING GENE LEADS TO FORMULATION OF THE MOST EFFECTIVE ANTI-AGEING COSMECEUTICAL: KLOTHO SKIN

Studies performed by Gail Humble M.D. & the Klotho Skin team. View official Study

2020

KLOTHO PROTEIN EXPRESSION IN SELF CONDITIONED MEDIA BY GENE TRANSFECTION IN PRIMARY SKIN CELLS

This paper summarizes the development by Klotho Skin of the first and only mesenchymal cell in humans which has been genetically modified to produce the Klotho protein and second generation growth factors. This paper summarizes our patented process. GAIL HUMBLE M.D., CellVi Bioscience

2021

EFFECTS OF ADVICELL-K ON THE EXPRESSION OF A PANEL OF SKIN RELEVANT GENES INCLUDING THOSE THAT ARE TELOMERE PROTECTIVE

GAIL HUMBLE M.D., KRYS BOJANOWSKI, PhD. View official Study

EFFECT OF ADVICELL-K ON THE OUTPUT OF PROSTAGLANDIN E2, TYPE I AND IV COLLAGEN BY THE HUMAN DERMAL FIBROBLASTS AFTER UVB EXPOSURE

The objective of this project was to explore the effect of the test material listed in Table I on the output of prostaglandin E2 (PGE2), type I and type IV collagen by human dermal fibroblasts. View official Study

EFFECT OF ADVICELL K ON THE OUTPUT OF PROSTAGLANDIN E2, TYPE I AND IV COLLAGEN BY THE HUMAN DERMAL FIBROBLASTS

The Klotho gene is an age suppressor gene originally discovered in a mouse strain. A defect in the Klotho gene expression in mice results in a syndrome resembling early ageing. View official Study

EFFECT OF ADVICELL K ON THE EXPRESSION OF A PANEL OF SKIN- ABSTRACT FINAL

In mice, the overexpression of the Klotho gene extends the life span, whereas mutations to the Klotho gene shorten the life span. View official Study

UTILIZING THE KLOTHO PROTEIN & 2ND GEN GF - ABSTRACT FINAL

The clinical sequelae of photodamage including wrinkling, pigmentary changes, texture change, laxity, and Transepidermal Water Loss (TEWL), can all result in the appearance of aging skin. View official Study

KLOTHO SKIN FORMULATIONS

For over 20 years, the founder and team at Klotho Skin have been studying the regenerative properties of the Klotho gene for implementation within skincare. Klotho Skin is dedicated to our decades-long and continued research alongside the responsibility to disclose all of our innovative findings.

RESEARCH GOALS

To create a cosmetic serum that will provide anti-aging benefits using a unique system of growth factors and the isolated Klotho protein.

To introduce a serum that goes beyond what has been available and uses second generation growth factors and the Klotho Protein to work on a genetic level.

To introduce a serum that is an anti-inflammatory as well as an antioxidant

TRUTHS

1. GROWTH FACTORS
Fibroblast, otherwise known as skin cells, are one of the first cells to respond to wound healing by secreting communication signals termed growth factors (GFs) to signal cellular proliferation and repair. As we age, the body produces less fibroblast cells and less growth factors which inhibits the body’s ability to defend itself against UV radiation and harmful environmental factors. Exposure to extreme environments of high temperatures, stress, poor nutrition, lack of sleep and causes free-radical damage and increased oxidative stress in cells.

2. KLOTHO GENE
The Klotho gene is expressed in many tissues and cell types, and its over-expression in mammals extends lifespan due to its ability to mimic the caloric restriction pathway of cells. The caloric restriction pathway moderately inhibits IGF-1 (insulin) signaling, which increases antioxidants and DNA repair while decreasing free radical damage and oxidative stress in cells. Further, the Klotho protein activates the FOXO forkhead transcription factors that are negatively regulated by insulin/IGF-1 signaling, which then facilitates oxidative stress resistance and directly protects cells from UVA and UVB radiation. In all, because of Klotho's ability to inhibit IGF-1 signaling and increase FOX01, cells develop increased resistance to oxidative stress as well as longevity and anti-ageing characteristics. Klotho expression declines by 60% in aging skin cells. View official Study

TECHNIQUES

Working with adult mesenchymal stem cells we used a gene promotor and a vector to up regulate the Klotho Gene of the stem cell. After verifying the cells viability, we then multiplied. The cell conditioned medium was then collected from these genetically modified cells and found we had second generation growth factors as well as the Klotho protein. There are no live cells in our face and eye serum, just the Klotho protein and the second generation growth factors.

RESULTS

Klotho Skin is the first to genetically modify adult mesenchymal stem cells and the Klotho protein in cell conditioned medium to produce elevated levels of the Klotho protein. This results in the production of second generation growth factors and Klotho protein.

Second generation growth factors and Klotho protein are an age preventative protein that supports cell renewal and wound repair. Second generation growth factors and Klotho protein has potent regenerative properties, making it critical for age prevention and age reversal. It is one oft the many growth factors that are isolated, purified and collected.

The second generation growth factors are combined with complementary ingredients to create a unique formulation that turns back the clock on your own fibroblasts. The formula is encapsulated in liposomal (fat-soluble) cells, allowing for the ingredients to overcome cellular absorption barriers and increase bioavailability.

Klotho Skin products utilize second generation growth factors to stimulate Klotho gene expression and assist the skin with developing more collagen, decreasing the breakdown of existing collagen, and TEWL (transepidermal water loss). Further, we are able to initiate an anti-inflammatory process and the reversal of oxidative stress to skin cells, creating the appearance of younger and healthier skin.

CLINICAL STUDIES

We are excited to share the results of these new studies and are currently in the process of applying for an NIH grant to fund future research!

HUMAN CLINICAL TRIALS

• We just received IRB approval to conduct a new clinical trial
• Study will be conducted by Dr. Pearl Grimes
• 20 subjects will use Klotho Skin for 3 consecutive months
• Biopsies will be performed to showcase histological results

CELLULAR STUDIES

• Study will be conducted by Amelia Technologies LLC
• Research will focus on the Klotho protein and how it can benefit cell damage caused by UVA exposure
• Results at the end of May!